Most research peptides are sold in vials of 2 mg, 5 mg, or 10 mg. Check your vial label. Common sizes include 5 mg (5,000 mcg) for BPC-157 and 10 mg (10,000 mcg) for TB-500. Enter this value in the Peptide Vial Amount field of the peptide reconstitution calculator.
Bacteriostatic water (BAC water) is the standard solvent for peptide reconstitution. The volume you choose sets your concentration. Adding 2 mL to a 5 mg vial gives 2,500 mcg/mL — a common starting point for most peptide research protocols. More water = lower concentration; less water = higher concentration per mL.
Use the Reconstitution tab to see your concentration in both mg/mL and mcg/mL instantly. This is the foundation of every subsequent peptide dose calculation. Write it down or bookmark the page for future reference.
Switch to the Dosage per Injection tab and enter your target dose in mcg. A common BPC-157 research protocol uses 250–500 mcg per injection. The calculator shows the exact mL to draw and the corresponding units on a U-100 insulin syringe.
Use a U-100 insulin syringe for precise small-volume peptide injections. The syringe has markings from 0–100 units, where 100 units = 1 mL. The syringe unit calculator converts your mL value directly to syringe markings — no mental math required.
Concentration (mcg/mL) = Vial Amount (mcg) ÷ BAC Water Added (mL)
Injection Volume (mL) = Desired Dose (mcg) ÷ Concentration (mcg/mL)
Syringe Units = Injection Volume (mL) × 100
| Peptide | Vial | BAC Water | Concentration | Dose Range | Category |
|---|---|---|---|---|---|
| BPC-157 | 5 mg | 2 mL | 2,500 mcg/mL | 250–500 mcg/dose | Tissue Repair |
| TB-500 (Thymosin β4) | 10 mg | 2 mL | 5,000 mcg/mL | 2,000–2,500 mcg/dose | Tissue Repair |
| CJC-1295 (no DAC) | 2 mg | 1 mL | 2,000 mcg/mL | 100–300 mcg/dose | GH Releasing |
| Ipamorelin | 2 mg | 2 mL | 1,000 mcg/mL | 200–300 mcg/dose | GH Releasing |
| Semaglutide | 5 mg | 2 mL | 2,500 mcg/mL | 250–2,400 mcg/week | GLP-1 / Metabolic |
| Tirzepatide | 5 mg | 2 mL | 2,500 mcg/mL | 2,500–15,000 mcg/week | GIP/GLP-1 / Metabolic |
| Sermorelin | 3 mg | 1 mL | 3,000 mcg/mL | 200–500 mcg/dose | GH Releasing |
| PT-141 (Bremelanotide) | 10 mg | 2 mL | 5,000 mcg/mL | 1,000–2,000 mcg/dose | Melanocortin |
| Selank | 5 mg | 5 mL | 1,000 mcg/mL | 250–500 mcg/dose | Nootropic |
| Semax | 30 mg | 30 mL | 1,000 mcg/mL | 300–600 mcg/dose | Nootropic |
| GHK-Cu (Copper Peptide) | 50 mg | 50 mL | 1,000 mcg/mL | 200–400 mcg/dose | Cosmetic / Repair |
| DSIP | 2 mg | 2 mL | 1,000 mcg/mL | 100–300 mcg/dose | Sleep / Recovery |
All values are for research reference only. Concentrations depend on actual BAC water volume used. Always verify using the reconstitution calculator above.
Bacteriostatic water is sterile water containing 0.9% benzyl alcohol as a preservative. The benzyl alcohol prevents bacterial growth, allowing reconstituted peptide solutions to remain stable for 4–6 weeks when refrigerated at 2–8°C. It is the preferred solvent for peptide reconstitution because it significantly extends the usability window compared to plain sterile water
There is no single correct amount — the ideal BAC water volume depends on your target dose and how precisely you need to measure it. Here are common configurations for a 5 mg peptide vial:
Add 1 mL BAC water → 5,000 mcg/mL (50 mcg per unit on U-100 syringe)
Add 2 mL BAC water → 2,500 mcg/mL (25 mcg per unit on U-100 syringe)
Add 5 mL BAC water → 1,000 mcg/mL (10 mcg per unit on U-100 syringe)
A good rule of thumb: choose the BAC water volume that makes your injection volume easy to measure. If your dose is 250 mcg and you use 2 mL BAC water in a 5 mg vial, you’ll draw exactly 10 units on a U-100 syringe — clean and precise. For a full guide on BAC water for peptides and storage, visit our reconstitution guide.
Wipe the rubber stopper of your peptide vial and BAC water vial with a 70% isopropyl alcohol swab. Allow to air-dry for 30 seconds before puncturing.
Draw your desired BAC water volume into a syringe. Inject slowly along the side wall of the vial — never directly onto the peptide powder, as forceful contact can denature fragile peptide bonds.
Gently swirl the vial in a circular motion until the powder is completely dissolved. Never shake a peptide vial. Mechanical agitation creates air bubbles and degrades peptide structure.
Store reconstituted vials at 2–8°C (standard refrigerator temperature). Keep away from direct light. A reconstituted peptide solution in bacteriostatic water remains stable for 4–6 weeks when stored correctly.
Lyophilized peptide powder is far more stable than reconstituted solution. Sealed vials under nitrogen or argon can be stored at -20°C (freezer) for 12–24 months. At room temperature, peptide powder is generally stable for 3–6 months if protected from heat and light. Once reconstituted, refrigerate immediately and use within 4–6 weeks.
Peptide dosages are expressed in several different units. Understanding the relationships between mcg, mg, IU, and how many units on an insulin syringe to draw is essential for accurate peptide dose calculation. Need help with mcg to mg conversion for peptides? See the chart below.
Both are units of mass, but at very different scales.
10 units = 0.10 mL
20 units = 0.20 mL
50 units = 0.50 mL
100 units = 1.00 mL
Some compounds — notably HGH and certain gonadotropins — are measured in International Units (IU) rather than mcg/mg. IU is a biological activity unit, not a mass unit, so conversion depends on the specific compound. For HGH, the general approximation is 1 mg ≈ 3 IU, though this varies by manufacturer.
| Dose (mcg) | Units Drawn (U-100) | Volume (mL) |
|---|---|---|
| 50 mcg | 1 unit | 0.01 mL |
| 100 mcg | 2 units | 0.02 mL |
| 150 mcg | 3 units | 0.03 mL |
| 200 mcg | 4 units | 0.04 mL |
| 250 mcg | 5 units | 0.05 mL |
| 300 mcg | 6 units | 0.06 mL |
| 500 mcg | 10 units | 0.10 mL |
| 1,000 mcg | 20 units | 0.20 mL |
Using a sterile syringe, draw your desired amount of bacteriostatic water (BAC water). Typical volumes range from 1mL to 3mL depending on concentration needs.
Slowly inject the BAC water into the peptide vial along the glass wall. Never spray directly onto the powder — let it trickle down gently.
Roll or gently swirl the vial between your fingers until the peptide is fully dissolved. Never shake vigorously as this damages the peptide structure.
Using the calculator above, determine how many units to draw for your desired dose. Pull back the syringe slowly for precision.
mcg/mL
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Get answers to the most common questions about peptide reconstitution, dosing, and calculations. Also check our in-depth guide on how to reconstitute BPC-157 and other research peptides.
